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Western blot(SDS PAGE) analysis of extracts from T47-D cells.Using Calpain small subunit 1Rabbit mAb [Z1I7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CAPNS1,CAPN4,CAPNS,CPNS1_HUMAN,Calpain small subunit 1
UniProtID :P04632
MASS(da) :28,316
MW(kDa) :28 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Calpain small subunit 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :T47-D cells
Function : Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity)..
Subcellular locationi :Cytoplasm. Cell membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.