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eIF2A Rabbit mAb [L8C9]Cat NO.: A34932

Western blot(SDS PAGE) analysis of extracts from Ramos cells.Using eIF2ARabbit mAb [L8C9] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :EIF2A,CDA02,MSTP004,MSTP089,EIF2A_HUMAN,Eukaryotic translation initiation factor 2A [Cleaved into: Eukaryotic translation initiation factor 2A, N-terminally processed]

UniProtID :Q9BY44

MASS(da) :64,990

MW(kDa) :65 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human eIF2A

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Ramos cells

Function : Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner..

Tissue specificity :Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.