* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from Ramos cells.Using eIF2ARabbit mAb [L8C9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF2A,CDA02,MSTP004,MSTP089,EIF2A_HUMAN,Eukaryotic translation initiation factor 2A [Cleaved into: Eukaryotic translation initiation factor 2A, N-terminally processed]
UniProtID :Q9BY44
MASS(da) :64,990
MW(kDa) :65 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human eIF2A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Ramos cells
Function : Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner..
Tissue specificity :Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.