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Western blot(SDS PAGE) analysis of extracts from HEK-293T cells .Using GOLPH4/GPP130Rabbit mAb [960D] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GOLIM4,GIMPC,GOLPH4,GPP130,GOLI4_HUMAN,Golgi integral membrane protein 4
UniProtID :O00461
MASS(da) :81,880
MW(kDa) :130 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GOLPH4/GPP130
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293T cells
Function : Plays a role in endosome to Golgi protein trafficking,mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi..
Subcellular locationi :Golgi apparatus, Golgi stack membrane,Single-pass type II membrane protein. Endosome membrane,Single-pass type II membrane protein. Membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.