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PBK/SPK Rabbit mAb [RARW]Cat NO.: A69904

Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using PBK/SPKRabbit mAb [RARW] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PBK,TOPK,TOPK_HUMAN,Lymphokine-activated killer T-cell-originated protein kinase

UniProtID :Q96KB5

MASS(da) :36,085

MW(kDa) :40 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PBK/SPK

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells 

Function : Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage..

Tissue specificity :Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.