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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using PBK/SPKRabbit mAb [RARW] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PBK,TOPK,TOPK_HUMAN,Lymphokine-activated killer T-cell-originated protein kinase
UniProtID :Q96KB5
MASS(da) :36,085
MW(kDa) :40 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PBK/SPK
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage..
Tissue specificity :Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.