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Western blot(SDS PAGE) analysis of extracts from HCT 116 CELLS.Using SA2Rabbit mAb [2V6X] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :STAG2,SA2,STAG2_HUMAN,Cohesin subunit SA-2
UniProtID :Q8N3U4
MASS(da) :141,326
MW(kDa) :141 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SA2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HCT 116 CELLS
Function : Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis..
Subcellular locationi :Nucleus. Chromosome. Chromosome, centromere.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.