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Western blot(SDS PAGE) analysis of extracts from Human fetal brain.Using GABARAPL2/GATE-16Rabbit mAb [5287] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GABARAPL2,FLC3A,GEF2,GBRL2_HUMAN,Gamma-aminobutyric acid receptor-associated protein-like 2 receptor-associated protein-like 2)
UniProtID :P60520
MASS(da) :13,667
MW(kDa) :14 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GABARAPL2/GATE-16
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal brain
Function : Ubiquitin-like modifier involved in intra-Golgi traffic (By similarity). Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation (By similarity). It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy (PubMed:20418806, PubMed:23209295). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:20418806, PubMed:23209295). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295)..
Tissue specificity :Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine..
Subcellular locationi :Cytoplasmic vesicle, autophagosome. Endoplasmic reticulum membrane. Golgi apparatus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.