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Western blot(SDS PAGE) analysis of extracts from EL4 cells.Using TNFRSF8/CD30 Rabbit mAb [4ZFZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TNFRSF8,CD30,D1S166E,TNR8_HUMAN,Tumor necrosis factor receptor superfamily member 8
UniProtID :P28908
MASS(da) :63,747
MW(kDa) :90, 120 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human TNFRSF8/CD30
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :EL4 cells
Function : Receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898)..
Tissue specificity :[Isoform 2]: Detected in alveolar macrophages (at protein level)..
Subcellular locationi :[Isoform 1]: Cell membrane,Single-pass type I membrane protein.,[Isoform 2]: Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.