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Phospholipase C beta 3/PLCB3 Rabbit mAb [50P3]Cat NO.: A56780

Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Phospholipase C beta 3/PLCB3Rabbit mAb [50P3] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PLCB3,PLCB3_HUMAN,1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

UniProtID :Q01970

MASS(da) :138,799

MW(kDa) :150 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Phospholipase C beta 3/PLCB3

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cells

Function : The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes..

Subcellular locationi :Cytoplasm. Membrane. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.