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Western blot(SDS PAGE) analysis of extracts from HCT 116 CELLS.Using NR2F2Rabbit mAb [T2L7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NR2F2,ARP1,TFCOUP2,COT2_HUMAN,COUP transcription factor 2
UniProtID :P24468
MASS(da) :45,571
MW(kDa) :46 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NR2F2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HCT 116 CELLS
Function : Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. May be required to establish ovary identity during early gonad development (PubMed:29478779)..
Tissue specificity :Ubiquitous. Expressed in the stromal cells of developing fetal ovaries (PubMed:29478779)..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.