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Western blot(SDS PAGE) analysis of extracts from Daudi cells .Using GAKRabbit mAb [5Z1P] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GAK,GAK_HUMAN,Cyclin-G-associated kinase
UniProtID :O14976
MASS(da) :143,191
MW(kDa) :160 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GAK
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Daudi cells
Function : Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1..
Tissue specificity :Ubiquitous. Highest in testis.
Subcellular locationi :Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.