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Western blot(SDS PAGE) analysis of extracts from NCI-H460 .Using LMAN1Rabbit mAb [4FPA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LMAN1,ERGIC53,F5F8D,LMAN1_HUMAN,Protein ERGIC-53
UniProtID :P49257
MASS(da) :57,549
MW(kDa) :55 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human LMAN1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : NCI-H460
Function : Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins..
Tissue specificity :Ubiquitous..
Subcellular locationi :Endoplasmic reticulum-Golgi intermediate compartment membrane,Single-pass type I membrane protein. Golgi apparatus membrane,Single-pass membrane protein. Endoplasmic reticulum membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.