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LMAN1 Rabbit mAb [4FPA]Cat NO.: A32016

Western blot(SDS PAGE) analysis of extracts from  NCI-H460 .Using LMAN1Rabbit mAb [4FPA] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :LMAN1,ERGIC53,F5F8D,LMAN1_HUMAN,Protein ERGIC-53

UniProtID :P49257

MASS(da) :57,549

MW(kDa) :55 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human LMAN1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : NCI-H460 

Function : Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins..

Tissue specificity :Ubiquitous..

Subcellular locationi :Endoplasmic reticulum-Golgi intermediate compartment membrane,Single-pass type I membrane protein. Golgi apparatus membrane,Single-pass membrane protein. Endoplasmic reticulum membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.