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Western blot(SDS PAGE) analysis of extracts from U266 cells.Using CD70 Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD70,CD27L,CD27LG,TNFSF7,CD70_HUMAN,CD70 antigen
UniProtID :P32970
MASS(da) :21,118
MW(kDa) :25-30 kDa
Form :Liquid
Purification :peptide affinity chromatography
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CD70
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U266 cells
Function : Cytokine which is the ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells..
Subcellular locationi :Membrane,Single-pass type II membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.