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Western blot(SDS PAGE) analysis of extracts from C2C12 cells.Using CD80 Rabbit mAb [V0PN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD80,CD28LG,CD28LG1,LAB7,CD80_HUMAN,T-lymphocyte activation antigen CD80
UniProtID :P33681
MASS(da) :33,048
MW(kDa) :50-75 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Mouse CD80
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :C2C12 cells
Function : Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.., (Microbial infection) Acts as a receptor for adenovirus subgroup B..
Tissue specificity :Expressed on activated B-cells, macrophages and dendritic cells.
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.