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SMAD5 Rabbit mAb [91K8]Cat NO.: A86995

Western blot(SDS PAGE) analysis of extracts from HEK293 cells .Using SMAD5Rabbit mAb [91K8] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SMAD5,MADH5,SMAD5_HUMAN,Mothers against decapentaplegic homolog 5

UniProtID :Q99717

MASS(da) :52,258

MW(kDa) :52 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SMAD5

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HEK293 cells 

Function : Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Tissue specificity :Ubiquitous.

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.