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P-cdc25C (S198) Rabbit pAbCat NO.: A75564

Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using P-cdc25C (S198) Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CDC25C,MPIP3_HUMAN,M-phase inducer phosphatase 3

UniProtID :P30307

MASS(da) :53,365

MW(kDa) :75 kDa

Form :Liquid

Purification :peptide affinity chromatography

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-cdc25C (Ser198)

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HT-29 cells

Function : Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.