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Western blot(SDS PAGE) analysis of extracts from Human heart .Using Cardiac Troponin IRabbit mAb [TL60] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TNNI3,TNNC1,TNNI3_HUMAN,Troponin I, cardiac muscle
UniProtID :P19429
MASS(da) :24,008
MW(kDa) :28 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cardiac Troponin I
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human heart
Function : Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.