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Western blot(SDS PAGE) analysis of extracts from BXPC-3 CELLS.Using PCTAIRE1Rabbit mAb [703T] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDK16,PCTAIRE1,PCTK1,CDK16_HUMAN,Cyclin-dependent kinase 16
UniProtID :Q00536
MASS(da) :55,716
MW(kDa) :56 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PCTAIRE1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :BXPC-3 CELLS
Function : Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro)..
Tissue specificity :Detected in pancreas islets (at protein level). Detected in brain and pancreas..
Subcellular locationi :Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cell junction, synapse, synaptosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.