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Flotillin 2/ESA Rabbit mAb [I174]Cat NO.: A55247

Western blot(SDS PAGE) analysis of extracts from HEK293 cells.Using Flotillin 2/ESARabbit mAb [I174] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :FLOT2,ESA1,M17S1,FLOT2_HUMAN,Flotillin-2

UniProtID :Q14254

MASS(da) :47,064

MW(kDa) :47 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Flotillin 2/ESA

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HEK293 cells

Function : May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

Tissue specificity :In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.

Subcellular locationi :Cell membrane,Peripheral membrane protein. Membrane, caveola,Peripheral membrane protein. Endosome. Membrane,Lipid-anchor.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.