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Western blot(SDS PAGE) analysis of extracts from HEK293 cells.Using Flotillin 2/ESARabbit mAb [I174] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FLOT2,ESA1,M17S1,FLOT2_HUMAN,Flotillin-2
UniProtID :Q14254
MASS(da) :47,064
MW(kDa) :47 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Flotillin 2/ESA
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293 cells
Function : May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Tissue specificity :In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Subcellular locationi :Cell membrane,Peripheral membrane protein. Membrane, caveola,Peripheral membrane protein. Endosome. Membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.