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DPF2/REQ Rabbit mAb [9V3D]Cat NO.: A79239

Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells .Using DPF2/REQRabbit mAb [9V3D] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :DPF2,BAF45D,REQ,UBID4,REQU_HUMAN,Zinc finger protein ubi-d4

UniProtID :Q92785

MASS(da) :44,155

MW(kDa) :44 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human DPF2/REQ

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :NIH/3T3 cells 

Function : Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed:28533407, PubMed:27775714). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed:28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed:20460684)..

Tissue specificity :Ubiquitous.

Subcellular locationi :Nucleus. Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.