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Western blot(SDS PAGE) analysis of extracts from HEK-293T cells .Using IFFO1Rabbit mAb [3SG7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IFFO1,IFFO,IFFO1_HUMAN,Non-homologous end joining factor IFFO1
UniProtID :Q0D2I5
MASS(da) :61,979
MW(kDa) :75 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human IFFO1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293T cells
Function : Nuclear matrix protein involved in the immobilization of broken DNA ends and the suppression of chromosome translocation during DNA double-strand breaks (DSBs) (PubMed:31548606). Interacts with the nuclear lamina component LMNA, resulting in the formation of a nucleoskeleton that relocalizes to the DSB sites in a XRCC4-dependent manner and promotes the immobilization of the broken ends, thereby preventing chromosome translocation (PubMed:31548606). Acts as a scaffold that allows the DNA repair protein XRCC4 and LMNA to assemble into a complex at the DSB sites (PubMed:31548606)..
Tissue specificity :Ubiquitously expressed..
Subcellular locationi :Nucleus. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.