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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using Rab 6ARabbit mAb [339I] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAB6A,RAB6,RAB6A_HUMAN,Ras-related protein Rab-6A
UniProtID :P20340
MASS(da) :23,593
MW(kDa) :24 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Rab 6A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Involved in COPI-independent retrograde transport from the Golgi to the ER (PubMed:25962623)..
Tissue specificity :Ubiquitous..
Subcellular locationi :Golgi apparatus membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.