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Aldolase Rabbit mAb [CRRL]Cat NO.: A73885

Western blot(SDS PAGE) analysis of extracts from  MCF-7 cells.Using AldolaseRabbit mAb [CRRL] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ALDOA,ALDA,ALDOA_HUMAN,Fructose-bisphosphate aldolase A

UniProtID :P04075

MASS(da) :39,420

MW(kDa) :39 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Aldolase

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : MCF-7 cells

Function : Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, may also function as scaffolding protein (By similarity)..

Subcellular locationi :Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.