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Western blot(SDS PAGE) analysis of extracts from HEK293 cells .Using GDF11 + GDF8/MyostatinRabbit mAb [F5UG] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MSTN,GDF8,GDF8_HUMAN,Growth/differentiation factor 8
UniProtID :O14793,O95390
MASS(da) :42,750
MW(kDa) :45 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GDF11 + GDF8/Myostatin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293 cells
Function : Acts specifically as a negative regulator of skeletal muscle growth..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.