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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cells.Using NCAM1Rabbit mAb [Y70J] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NCAM1,NCAM,NCAM1_HUMAN,Neural cell adhesion molecule 1
UniProtID :P13591
MASS(da) :94,574
MW(kDa) :140-180 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NCAM1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cells
Function : This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc., (Microbial infection) Acts as a receptor for rabies virus.., (Microbial infection) Acts as a receptor for Zika virus..
Subcellular locationi :[Isoform 1]: Cell membrane,Single-pass type I membrane protein.,[Isoform 2]: Cell membrane,Single-pass type I membrane protein.,[Isoform 3]: Cell membrane,Lipid-anchor, GPI-anchor.,[Isoform 4]: Cell membrane,Lipid-anchor, GPI-anchor.,[Isoform 5]: Secreted.,[Isoform 6]: Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.