TNF Receptor II Rabbit mAb [5523]Cat NO.: A85064

Protein names ：TNFRSF1B,TNFBR,TNFR2,TNR1B_HUMAN,Tumor necrosis factor receptor superfamily member 1B [Cleaved into: Tumor necrosis factor receptor superfamily member 1b, membrane form,Tumor necrosis factor-binding protein 2 ]

UniProtID ：P20333

MASS(da) ：48,291

MW(kDa) ：73 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TNF Receptor II

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Jurkat cells

Function ： Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity..

Subcellular locationi ：[Isoform 1]: Cell membrane,Single-pass type I membrane protein.,[Isoform 2]: Secreted.,[Tumor necrosis factor-binding protein 2]: Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.