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Western blot(SDS PAGE) analysis of extracts from Human hypothalamus.Using BrevicanRabbit mAb [J04U] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BCAN,BEHAB,CSPG7,UNQ2525/PRO6018,PGCB_HUMAN,Brevican core protein
UniProtID :Q96GW7
MASS(da) :99,118
MW(kDa) :53 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Brevican
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human hypothalamus
Function : May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.
Tissue specificity :Expressed in the retina, specifically in the inner nuclear layer, inner plexiform layer and ganglion cell layer (at protein level)..
Subcellular locationi :[Isoform 1]: Secreted, extracellular space, extracellular matrix.,[Isoform 2]: Membrane,Lipid-anchor, GPI-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.