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Western blot(SDS PAGE) analysis of extracts from SK-MEL-2.Using MAGEA10Rabbit mAb [UV6G] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAGEA10,MAGE10,MAGAA_HUMAN,Melanoma-associated antigen 10
UniProtID :P43363
MASS(da) :40,780
MW(kDa) :65 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MAGEA10
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : SK-MEL-2
Function : Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue specificity :Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for spermatogonia, spermatocytes and placenta..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.