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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ALDH1L1Rabbit mAb [914C] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ALDH1L1,FTHFD,AL1L1_HUMAN,Cytosolic 10-formyltetrahydrofolate dehydrogenase
UniProtID :O75891
MASS(da) :98,829
MW(kDa) :94 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ALDH1L1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Cytosolic 10-formyltetrahydrofolate dehydrogenase that catalyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide (PubMed:19933275, PubMed:21238436). May also have an NADP(+)-dependent aldehyde dehydrogenase activity towards formaldehyde, acetaldehyde, propionaldehyde, and benzaldehyde (By similarity)..
Tissue specificity :Highly expressed in liver, pancreas and kidney..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.