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Western blot(SDS PAGE) analysis of extracts from HuH-6 cells.Using ChREBP Rabbit pAb at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MLXIPL,BHLHD14,MIO,WBSCR14,MLXPL_HUMAN,Carbohydrate-responsive element-binding protein
UniProtID :Q9NP71
MASS(da) :93,073
MW(kDa) :110 kDa
Form :Liquid
Purification :peptide affinity chromatography
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human ChREBP
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HuH-6 cells
Function : Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3' (By similarity)..
Tissue specificity :Expressed in liver, heart, kidney, cerebellum and intestinal tissues.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.