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ATG10 Rabbit mAb [Y9CT]Cat NO.: A94692

Western blot(SDS PAGE) analysis of extracts from  K562 cells.Using ATG10Rabbit mAb [Y9CT] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ATG10,APG10L,PP12616,ATG10_HUMAN,Ubiquitin-like-conjugating enzyme ATG10

UniProtID :Q9H0Y0

MASS(da) :25,279

MW(kDa) :28 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ATG10

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : K562 cells

Function : E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.