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Western blot(SDS PAGE) analysis of extracts from MDA-MB-435.Using Tartrate Resistant Acid PhosphataseRabbit mAb [1Y7B] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACP5,PPA5_HUMAN,Tartrate-resistant acid phosphatase type 5
UniProtID :P13686
MASS(da) :36,599
MW(kDa) :42 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Tartrate Resistant Acid Phosphatase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MDA-MB-435
Function : Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
Subcellular locationi :Lysosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.