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Western blot(SDS PAGE) analysis of extracts from HuvEc cells .Using Factor VIIIRabbit mAb [YQ06] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :F8,F8C,FA8_HUMAN,Coagulation factor VIII [Cleaved into: Factor VIIIa heavy chain, 200 kDa isoform,Factor VIIIa heavy chain, 92 kDa isoform,Factor VIII B chain,Factor VIIIa light chain]
UniProtID :P00451
MASS(da) :267,009
MW(kDa) :166,210-280,330 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Factor VIII
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HuvEc cells
Function : Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
Subcellular locationi :Secreted, extracellular space.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.