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Factor VIII Rabbit mAb [YQ06]Cat NO.: A57761

Western blot(SDS PAGE) analysis of extracts from HuvEc cells .Using Factor VIIIRabbit mAb [YQ06] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :F8,F8C,FA8_HUMAN,Coagulation factor VIII [Cleaved into: Factor VIIIa heavy chain, 200 kDa isoform,Factor VIIIa heavy chain, 92 kDa isoform,Factor VIII B chain,Factor VIIIa light chain]

UniProtID :P00451

MASS(da) :267,009

MW(kDa) :166,210-280,330 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Factor VIII

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HuvEc cells 

Function : Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

Subcellular locationi :Secreted, extracellular space.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.