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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using PRA1Rabbit mAb [W39X] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RABAC1,PRA1,PRAF1,PRAF1_HUMAN,Prenylated Rab acceptor protein 1
UniProtID :Q9UI14
MASS(da) :20,648
MW(kDa) :21 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PRA1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI..
Tissue specificity :Ubiquitous. Strongest expression found in placenta, pituitary gland, kidney, lung and stomach..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cytoplasm. Golgi apparatus. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.