P-Cofilin (S3) Rabbit pAbCat NO.: A50388

Protein names ：CFL1,CFL,COF1_HUMAN,Cofilin-1

UniProtID ：P23528

MASS(da) ：18,502

MW(kDa) ：19 kDa

Form ：Liquid

Purification ：peptide affinity chromatography

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-Cofilin (Ser3)

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：mitotic C6 cells

Function ： Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity)..

Tissue specificity ：Widely distributed in various tissues.

Subcellular locationi ：Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane,Peripheral membrane protein,Cytoplasmic side. Cell projection, lamellipodium membrane,Peripheral membrane protein,Cytoplasmic side. Cell projection, lamellipodium. Cell projection, growth cone. Cell projection, axon.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.