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Alpha B Crystallin Rabbit mAb [3G47]Cat NO.: A27695

Western blot(SDS PAGE) analysis of extracts from human fetal heart .Using Alpha B CrystallinRabbit mAb [3G47] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CRYAB,CRYA2,HSPB5,CRYAB_HUMAN,Alpha-crystallin B chain -crystallin)

UniProtID :P02511

MASS(da) :20,159

MW(kDa) :20 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Alpha B Crystallin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human fetal heart 

Function : May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity)..

Tissue specificity :Lens as well as other tissues (PubMed:838078, PubMed:2387586). Expressed in myocardial tissue (PubMed:28493373)..

Subcellular locationi :Cytoplasm. Nucleus. Secreted. Lysosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.