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Western blot(SDS PAGE) analysis of extracts from Jurkat cells whole cell lysates treated with 25uM etoposide for 5 hours.Using Caspase-2Rabbit mAb [XX5D] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CASP2,ICH1,NEDD2,CASP2_HUMAN,Caspase-2 [Cleaved into: Caspase-2 subunit p18,Caspase-2 subunit p13,Caspase-2 subunit p12]
UniProtID :P42575
MASS(da) :50,685
MW(kDa) :49 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Caspase-2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells whole cell lysates treated with 25uM etoposide for 5 hours
Function : Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321). Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321)..
Tissue specificity :Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.