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Western blot(SDS PAGE) analysis of extracts from MDA MB 435.Using Extracellular matrix protein 1Rabbit mAb [L91T] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ECM1,ECM1_HUMAN,Extracellular matrix protein 1
UniProtID :Q16610
MASS(da) :60,674
MW(kDa) :61 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Extracellular matrix protein 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MDA MB 435
Function : Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity..
Tissue specificity :Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin,wide expression is observed throughout the dermis with minimal expression in the epidermis..
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.