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GCLC Rabbit mAb [2GZ0]Cat NO.: A38041

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using GCLCRabbit mAb [2GZ0] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :GCLC,GLCL,GLCLC,GSH1_HUMAN,Glutamate--cysteine ligase catalytic subunit

UniProtID :P48506

MASS(da) :72,766

MW(kDa) :73 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GCLC

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.