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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using GCLCRabbit mAb [2GZ0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GCLC,GLCL,GLCLC,GSH1_HUMAN,Glutamate--cysteine ligase catalytic subunit
UniProtID :P48506
MASS(da) :72,766
MW(kDa) :73 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GCLC
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.