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Western blot(SDS PAGE) analysis of extracts from HEK293T cells .Using NicastrinRabbit mAb [5WPM] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NCSTN,KIAA0253,UNQ1874/PRO4317,NICA_HUMAN,Nicastrin
UniProtID :Q92542
MASS(da) :78,411
MW(kDa) :100-125 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Nicastrin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293T cells
Function : Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:10993067, PubMed:12679784, PubMed:25043039, PubMed:26280335, PubMed:30598546, PubMed:30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels..
Tissue specificity :Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676)..
Subcellular locationi :Membrane,Single-pass type I membrane protein. Cytoplasmic vesicle membrane,Single-pass type I membrane protein. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.