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Western blot(SDS PAGE) analysis of extracts from A-204 cells.Using COL11A1 Rabbit mAb [12ZC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COL11A1,COLL6,COBA1_HUMAN,Collagen alpha-1 chain
UniProtID :P12107
MASS(da) :181,065
MW(kDa) :250 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human COL11A1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A-204 cells
Function : May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Tissue specificity :Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.