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Western blot(SDS PAGE) analysis of extracts from A431 cells.Using TRIM29Rabbit mAb [D5I3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TRIM29,ATDC,TRI29_HUMAN,Tripartite motif-containing protein 29
UniProtID :Q14134
MASS(da) :65,835
MW(kDa) :66 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TRIM29
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells
Function : Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation..
Tissue specificity :Expressed in placenta, prostate and thymus..
Subcellular locationi :Cytoplasm. Lysosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.