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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using KDM5C / Jarid1C / SMCXRabbit mAb [Q671] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KDM5C,DXS1272E,JARID1C,SMCX,XE169,KDM5C_HUMAN,Lysine-specific demethylase 5C demethylase 5C)
UniProtID :P41229
MASS(da) :175,720
MW(kDa) :180 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human KDM5C / Jarid1C / SMCX
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:28262558). Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity)..
Tissue specificity :Expressed in all tissues examined. Highest levels found in brain and skeletal muscle..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.