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Western blot(SDS PAGE) analysis of extracts from SK-N-SH cells.Using COL1A1 mouse mAb[1C8E] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COL1A1,CO1A1_HUMAN,Collagen alpha-1 chain
UniProtID :P02452
MASS(da) :138,911
MW(kDa) :220 kDa
Form :Liquid
Purification :Protein A purification
Host :mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human COL1A1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SK-N-SH cells
Function : Type I collagen is a member of group I collagen (fibrillar forming collagen).
Tissue specificity :Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.