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Western blot(SDS PAGE) analysis of extracts from HEK-293T cells.Using LRP6Rabbit mAb [BWGD] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LRP6,LRP6_HUMAN,Low-density lipoprotein receptor-related protein 6
UniProtID :O75581
MASS(da) :180,429
MW(kDa) :18,36 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human LRP6
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293T cells
Function : Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity)..
Tissue specificity :Widely coexpressed with LRP5 during embryogenesis and in adult tissues.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Endoplasmic reticulum. Membrane raft.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.