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Nkx2.5 Rabbit mAb [898C]Cat NO.: A54203

Western blot(SDS PAGE) analysis of extracts from HEK-293T cells .Using Nkx2.5Rabbit mAb [898C] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NKX2-5,CSX,NKX2.5,NKX2E,NKX25_HUMAN,Homeobox protein Nkx-2.5

UniProtID :P52952

MASS(da) :34,918

MW(kDa) :30-42 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Nkx2.5

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HEK-293T cells 

Function : Transcription factor required for the development of the heart and the spleen (PubMed:22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed:22849347, PubMed:26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed:22560297)..

Tissue specificity :Expressed only in the heart.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.