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PSGL-1 Rabbit mAb [57G3]Cat NO.: A19525

Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using PSGL-1Rabbit mAb [57G3] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SELPLG,SELPL_HUMAN,P-selectin glycoprotein ligand 1

UniProtID :Q14242

MASS(da) :43,201

MW(kDa) :120 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PSGL-1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cells 

Function : A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.., (Microbial infection) Acts as a receptor for enterovirus 71..

Tissue specificity :Expressed on neutrophils, monocytes and most lymphocytes.

Subcellular locationi :Membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.