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COMT Rabbit mAb [54S9]Cat NO.: A84677

Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using COMT Rabbit mAb [54S9] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :COMT,COMT_HUMAN,Catechol O-methyltransferase

UniProtID :P21964

MASS(da) :30,037

MW(kDa) :24, 28 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human COMT

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K-562 cells

Function : Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol..

Tissue specificity :Brain, liver, placenta, lymphocytes and erythrocytes.

Subcellular locationi :[Isoform Soluble]: Cytoplasm.,[Isoform Membrane-bound]: Cell membrane,Single-pass type II membrane protein,Extracellular side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.