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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using CPT1A Rabbit mAb [LX24] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CPT1A,CPT1,CPT1A_HUMAN,Carnitine O-palmitoyltransferase 1, liver isoform
UniProtID :P50416
MASS(da) :88,368
MW(kDa) :88 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CPT1A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed:9691089, PubMed:11350182, PubMed:14517221). Plays an important role in hepatic triglyceride metabolism (By similarity)..
Tissue specificity :Strong expression in kidney and heart, and lower in liver and skeletal muscle.
Subcellular locationi :Mitochondrion outer membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.