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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using RasRabbit mAb [SBH1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HRAS,HRAS1,RASH_HUMAN,GTPase HRas [Cleaved into: GTPase HRas, N-terminally processed]
UniProtID :P01112
MASS(da) :21,298
MW(kDa) :21 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Ras
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151)..
Tissue specificity :Widely expressed..
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.