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RAPGEF5 Rabbit mAb [7Z9H]Cat NO.: A91731

Western blot(SDS PAGE) analysis of extracts from 293T cells.Using RAPGEF5Rabbit mAb [7Z9H] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :RAPGEF5,GFR,KIAA0277,MRGEF,RPGF5_HUMAN,Rap guanine nucleotide exchange factor 5

UniProtID :Q92565

MASS(da) :67,733

MW(kDa) :68 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human RAPGEF5

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cells

Function : Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation..

Tissue specificity :Widely expressed with highest levels in brain.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.