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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using RAPGEF5Rabbit mAb [7Z9H] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAPGEF5,GFR,KIAA0277,MRGEF,RPGF5_HUMAN,Rap guanine nucleotide exchange factor 5
UniProtID :Q92565
MASS(da) :67,733
MW(kDa) :68 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human RAPGEF5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation..
Tissue specificity :Widely expressed with highest levels in brain.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.